The In-Situ One Health Problem Based Learning was held from 7th to 9th October and also from 14th to 16th October 2016. The program was held at the Centre for Livestock Farming, Lenggong, Perak. The objective of the program was to expose students to the application of theories learned in class at the pre-clinical level to the problem investigation used by the One Health concept. Other than that, this program also aimed to introduce in-situ Problem Based Learning (PBL) experience to a more diverse group of students covering medical, veterinary and ecosystem health. 160 participants from various courses namely Animal Conservation, Biology, Biomedical Science, Veterinary Medicine, Public Health, Environmental Health, Medicine, Pharmacy, and Plant Science were involved in this program. PBL cases were designed in this program to integrate the knowledge of One Health with inputs and participation from local and international facilitators. At the end of the session, the participants acquired knowledge about One Health and were able to relate concepts of PBL in the field compared to the knowledge they acquired in a classroom setting. Participants also had the opportunity to learn and understand more about the real-life situation faced by the respected agencies during a zoonotic disease outbreak and safety measures that need to be taken to curb and prevent the occurrence of the diseases.