The Training of Trainers (ToT) and Simulation of in-situ Problem Based Learning (PBL) Cases Workshop was held at the Copthorne Hotel and Jaya Dairy Farm, Cameron Highlands, Pahang from 22nd to 25th of August 2016. 31 participants from different disciplines such as Veterinary Health, Medicine, and Environmental Health presented. The workshop aimed to prepare facilitators to meet the requirements to conduct the in-situ PBL, assess the suitability of the current One Health PBL cases, and also to train new facilitators from other related disciplines in facilitating the in-situ PBL session. Engaged in scenarios created, participants were actively involved in activities which prompted their skills that will be needed in real-life situations. In relation to the workshop, most participants acknowledged the importance of the knowledge and workshops in order to develop a better and greater One Health future workforce.