Organised in Ramada Plaza Melaka the One Health Problem Based Case Development Workshop was held from 14th to 18th April 2017. A total of 35 participants attended the workshop came from different background of veterinary, public health and environment. The program began with the Welcoming Remarks from Prof. Dr. Latiffah Hassan and the Introduction and Overview of the workshop by the project leader, Assoc. Prof. Dr. Jalila Abu. Several lectures were given throughout the workshop by several speakers such as Assoc. Prof. Dr. Siti Suri Arshad from Universiti Putra Malaysia, Dr. Janetrix Hellen Amuguni from Tufts University, United States and Prof. Dr. Gregory C. Sales from University of Minnesota, United States. With eight facilitators assisting the participants, the workshop aimed to develop scenarios and cases based on zoonotic diseases of local and Southeast Asia SEA importance given specific One Health learning objectives and learning issues and to compile both a student and facilitators guide with the aim of publishing the second One Health problem-based learning book (e-book) that can be used locally and internationally for undergraduate and postgraduate education. The workshop ended with sharing session of the cases developed from this 4-day workshop and critical reviewed by Dr. Janetrix Hellen Amuguni and Assoc. Prof. Dr. Jalila Abu.