From 29th June until 1st July 2018, Regional Training of Trainers (ToT): One Health Problem Based Book 2 was held in Hotel Perdana, Kota Bharu, Kelantan. The activity aims to train facilitators on the use of One Health PBL book 1 and 2 in teaching undergraduate students, as well as to improve the quality of PBL cases from both books based on feedbacks. The activity is built upon past activities on the development of OH PBL book 1. Dr. Hellen Amuguni from Tufts University, United States was again invited to be the trainer for the activity. Twenty-eight academicians that have different disciplines and from various universities such as UniSZA, UPM, UNIMAS, UMK, IIUM, and UKM attended the activity.