The Online Learning Design and Development Workshop was held in Pullman Putrajaya Lakeside Hotel from 18th to 21st July 2016. 28 participants attended the workshop came from various institutions and fields of expertise. They were first introduced to One Health before being engaged into the main content of the workshop. This 4-day workshop intended to improve the participants’ current course design and to develop a new instructional course that will be effective to each course. Moreover, online learning can create student-centered and flexible learning methods and effective teaching deliveries that use the information and communication technologies. Academics from 6 universities namely University Malaya, Universiti Kebangsaan Malaysia, Universiti Putra Malaysia, Universiti Teknologi MARA, Cyberjaya University College of Medical Sciences and Universiti Sains Islam that participated in this workshop gave positive feedbacks. Through this workshop, the participants had better understanding of the instructional course and e-learning principles, with the hope that this might change the way they design their lessons in the future