Educating and implementing One Health concept to the younger workforce is one of the key elements in the One Health approach. This can be done through implementing the One Health concept through the existing curriculum in institutions. In tandem with the idea, a workshop in Mapping One Health Implementation in Curriculum was held from 20th to 23rd October in Ramada Plaza Hotel, Melaka. Aimed to document the implementation of One Health core competencies according to the Malaysian Universities Curriculum, 33 participants attended the workshop who came from a variety of expertise related to the Human-Animal-Environmental Health. Two academicians from MyOHUN members; Dr Razitasham Safii from Universiti Malaysia Sarawak, and Dr Rozaihan Mansor from Universiti Putra Malaysia were invited to share their experience on the implementation of One Health core competencies in their courses. Dr Karin Hamilton from the University of Minnesota, USA guided and facilitated the discussions on mapping One Health competencies and concepts into the curriculum and courses. The compilation of information on One Health core competencies progressed well since most institutions involved have already documented the implementation of One Health core competencies in their courses.